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Plasmid Construction pGEX-4T-1 was modified with a 6XHis tag in front of a GST tag at the N-terminus, and the thrombin protease cleave sequence was replaced by the TEV protease cleave site by a one-step site-directed deletion and insertion method [59]

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And then a few other neuroanatomists like Ted Jones is there, but he's like the neuroanatomist of my generation